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Diagram of an AlphaScreen assay strategy to follow the generation of a reaction product due to enzymatic activity. Here, the activity of a kinase (represented in green) is followed by the phosphorylation of a biotinylated peptide substrate (represented as a green ribbon).A phospho-specific antibody (red), conjugated to an acceptor bead, recognizes the phosphorylated …
أكمل القراءةMore Details. The Malachite Green Phosphate Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be …
أكمل القراءةScreening of Trametes strains for efficient decolorization of malachite green at high temperatures and ionic concentrations February 2014 International Biodeterioration & …
أكمل القراءةThe present study was designed to screen potential antiparasitic medicinal plants based on our previous studies, and comprehensively evaluate in vitro and in vivo anti-ich activity of selected plant extracts. The screening results showed that the methanol extract of Psoralea corylifolia had the highest activity against I. multifiliis theronts.
أكمل القراءة· Malachite green (MG) was decolorized by laccase (LacA) of white-rot fungus Cerrena sp. with strong decolorizing ability. Decolorization conditions were optimized with response surface methodology. A highly significant quadratic …
أكمل القراءة· A total of 120 rhizobacteria were isolated from seven different tea estates of Darjeeling, West Bengal, India. Based on a functional screening of in vitro plant growth-promoting (PGP) activities ...
أكمل القراءة· Trametes (synonym Coriolous) species are the most important sources for laccases with attractive properties. Several Trametes species were screened fo…
أكمل القراءة· In preliminary study, a malachite green assay (Roengsamran et al., 2000), a conventional radioassay (Coste and Grondin, 1995) and SPA (GE Healthcare, USA) were applied for PDE inhibitory activity determination of some plant extracts. The malachite green assay is based on coupling enzymatic reaction between PDEs and 5′-nucleotidase.
أكمل القراءة· Establishment of a transgenic yeast screening system for estrogenicity and identification of the anti-estrogenic activity of malachite green. ... Development of a fish reporter gene system for the assessment of estrogenic compounds and sewage treatment plant effluents. Ackermann GE, Brombacher E, Fent K. Environ Toxicol Chem, (9) ...
أكمل القراءة· Plants' usage as medicines goes back to early man. It can be said with certainty that the great civilizations of ancient nations such as China, India and North Africa have provided written evidence of man's ingenuity in utilizing plants for the treatment of a wide variety of diseases and as food [].Medicinal plants or herbs are considered to be plant's chemical …
أكمل القراءة· Aubert, S. & Schwitzguébel, J.-P. Screening of plant species for the phytotreatment of wastewater containing sulphonated anthraquinones. Water Res. 38, 3569–3575 (2004). CAS Article Google Scholar
أكمل القراءةBioAssay Systems' ATPase/GTPase Assay Kit (DATG-100) determines ATPase/GTPase activity. The kit features a single reagent for accurate enzyme activity determination. Malachite green reagent forms a stable dark green …
أكمل القراءةThe malachite green assay is often used for measuring the presence of inorganic mono-phosphate concentrations. ... Screening plants for PDE inhibitory activity may help to …
أكمل القراءة· Malachite green (MG) is a popular cationic triphenylmethane dye and has widely been used in the silk, dye, leather and textile industry 1.Despite its prohibited use in aquaculture in many ...
أكمل القراءةMalachite Green Assay. The Malachite Green Assay Kit is based on quantification of the green complex formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits ...
أكمل القراءة· The screening platform for FtsZ inhibitors was established for controlling plant bacterial diseases. • The natural 4'-demethylepipodophyllotoxin (DMEP) exhibits good bioactivities against Xanthomonas oryzae pv. oryzae. The natural DMEP can serve as a novel lead compound for exploring highly bioactive FtsZ inhibitors.
أكمل القراءة· Copper oxide minerals composed of carbonates consume high quantities of leaching reagent. The present research proposes an alternative procedure for malachite leaching (Cu2CO3(OH)2) through the use of only compound, ammonium hydroxide (NH4OH). Preliminary studies were also carried out for the dissolution of malachite in an acid system. The variables …
أكمل القراءة· From a chemical viewpoint, chitin is a poly(β-(1-4)-N-acetyl-d-glucosamine) with β(1→4) linkages [], and it is considered to have a cellulose-like structure, where the hydroxyl group in the C2 position has been replaced by an acetamido group (Figure 1).Depending on the orientation of polysaccharide chains, chitin displays three polymorphs, denoted as α, β and γ [].
أكمل القراءةMalachite green (MG) has been used globally in aquaculture but is not registered for use in food-producing animals in the European Union. The European Commission requested EFSA to evaluate whether a reference point for action (RPA) of 2 μg/kg for the sum of MG and its major metabolite leucomalachite green (LMG) is adequate to protect public health.
أكمل القراءة· Reif et al. fused the malachite green aptamer, ... Although GFP can be used as a marker for the rapid visual screening of transgenic plants 36,37, this approach requires constructing a fusion ...
أكمل القراءة· The malachite green dye stock solution was prepared according to Pegan et al. . In short, 300 mL of 18 M sulfuric acid stock was mixed with 1.5 L of water and then cooled down to room temperature. Malachite green powder (2.2 g) was then added and mixed by stirring with a magnetic stir bar and stir plate.
أكمل القراءة· Screening, degrading characteristics and community structure of a high molecular weight polycyclic aromatic hydrocarbon-degrading bacterial consortium from contaminated soil. Journal of Environmental Sciences, 2010, 22(10): 1576-1585. 51. 。
أكمل القراءةGC/MS screening of buckthorn phytochemicals and their use to synthesize ZnO nanoparticles for photocatalytic degradation of malachite green dye in water Ibrahem M. A. Hasan ... approach for improved photocatalytic activity and antibacterial properties of zinc sulfide nanoparticles using plant extract of Acalypha indica and Tridax procumbens ...
أكمل القراءةAlphaScreen technology has been routinely utilized in high throughput screening assays to quantify analyte accumulation or depletion, bimolecular interactions, and post-translational modifications. The high signal-to-background, dynamic range and sensitivity ...
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